384-channel parallel microfluidic cytometer for rare-cell screening

384-channel parallel microfluidic cytometer for rare-cell screening

We have constructed a 384-channel parallel microfluidic cytometer (PMC). The multichannel structure permits 384 distinctive samples for a cell-based display screen to be learn out in roughly 6-10 min, about 30-times the velocity of a traditional fluorescence-activated cytometer system (FACS). This structure additionally permits the sign integration time to be different over a bigger vary …

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Rapid, simple influenza RNA extraction from nasopharyngeal samples

Rapid, simple influenza RNA extraction from nasopharyngeal samples

This report describes the event and pre-clinical testing of a brand new, random-access RNA pattern preparation system (TruTip) for nasopharyngeal samples. The system is predicated on a monolithic, porous nucleic acid binding matrix embedded inside an aerosol-resistant pipette tip and might be operated with single or multi-channel pipettors. Equivalent extraction efficiencies had been obtained between automated QIAcube and …

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Evaluation of techniques for performing cellular isolation and preservation during microgravity conditions

Evaluation of techniques for performing cellular isolation and preservation during microgravity conditions

Genomic and epigenomic research require the exact switch of microliter volumes amongst differing types of tubes as a way to purify DNA, RNA, or protein from organic samples and subsequently carry out analyses of DNA methylation, RNA expression, and chromatin modifications on a genome-wide scale. Epigenomic and transcriptional analyses of human blood cells, for instance, …

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